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uls™ microrna labeling kit cy3  (Kreatech Diagnostics)
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Kreatech Diagnostics uls™ microrna labeling kit cy3
Cellular entry, nuclear uptake and chromosomal association of fluorescently labelled DNAfs and Cfs. NIH3T3 cells (10×104) were treated with DNAfs labelled with <t>ULS</t> (red) and Cfs dual-labelled with ULS (red) and ATTO-TEC (green) (10 ng DNA in all experiments). (A) Intracellular fate of DNAfs at 30 min as analysed by LSCM. Numerous fine fluorescent particles are seen in the cytoplasm and in the nucleus. DIC, DAPI and ULS pictures are represented in different panels. (B) Intracellular fate of Cfs at 6 h as analysed by LSCM. Presence of dual-labelled Cfs in the cytoplasm and nuclei are clearly seen. The red and green signals appear yellow in colour when the images are overlapped. (C) Kinetics of nuclear uptake of fluorescently labelled DNAfs and Cfs analysed by LSCM. Fifty nuclei were analysed at each time-point and the percentage of positive nuclei was recorded. Nuclei containing at least two fluorescent spots were considered as positive. (D and E) Association of fluorescently labelled DNAfs (D) and Cfs (E) with chromosomes of treated cells. NIH3T3 cells were treated with labelled DNAfs and Cfs and metaphase spreads were prepared 6 h after treatment and analysed by fluorescence microscopy. Note that the labelled DNA particles are considerably smaller in size than Cfs particles.
Uls™ Microrna Labeling Kit Cy3, supplied by Kreatech Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/uls+rna+labeling+kit/pmc05779614-70-13-19?v=Kreatech+Diagnostics
Average 90 stars, based on 1 article reviews
uls™ microrna labeling kit cy3 - by Bioz Stars, 2026-08
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Cellular entry, nuclear uptake and chromosomal association of fluorescently labelled DNAfs and Cfs. NIH3T3 cells (10×104) were treated with DNAfs labelled with ULS (red) and Cfs dual-labelled with ULS (red) and ATTO-TEC (green) (10 ng DNA in all experiments). (A) Intracellular fate of DNAfs at 30 min as analysed by LSCM. Numerous fine fluorescent particles are seen in the cytoplasm and in the nucleus. DIC, DAPI and ULS pictures are represented in different panels. (B) Intracellular fate of Cfs at 6 h as analysed by LSCM. Presence of dual-labelled Cfs in the cytoplasm and nuclei are clearly seen. The red and green signals appear yellow in colour when the images are overlapped. (C) Kinetics of nuclear uptake of fluorescently labelled DNAfs and Cfs analysed by LSCM. Fifty nuclei were analysed at each time-point and the percentage of positive nuclei was recorded. Nuclei containing at least two fluorescent spots were considered as positive. (D and E) Association of fluorescently labelled DNAfs (D) and Cfs (E) with chromosomes of treated cells. NIH3T3 cells were treated with labelled DNAfs and Cfs and metaphase spreads were prepared 6 h after treatment and analysed by fluorescence microscopy. Note that the labelled DNA particles are considerably smaller in size than Cfs particles.

Journal: Journal of biosciences

Article Title: Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes

doi:

Figure Lengend Snippet: Cellular entry, nuclear uptake and chromosomal association of fluorescently labelled DNAfs and Cfs. NIH3T3 cells (10×104) were treated with DNAfs labelled with ULS (red) and Cfs dual-labelled with ULS (red) and ATTO-TEC (green) (10 ng DNA in all experiments). (A) Intracellular fate of DNAfs at 30 min as analysed by LSCM. Numerous fine fluorescent particles are seen in the cytoplasm and in the nucleus. DIC, DAPI and ULS pictures are represented in different panels. (B) Intracellular fate of Cfs at 6 h as analysed by LSCM. Presence of dual-labelled Cfs in the cytoplasm and nuclei are clearly seen. The red and green signals appear yellow in colour when the images are overlapped. (C) Kinetics of nuclear uptake of fluorescently labelled DNAfs and Cfs analysed by LSCM. Fifty nuclei were analysed at each time-point and the percentage of positive nuclei was recorded. Nuclei containing at least two fluorescent spots were considered as positive. (D and E) Association of fluorescently labelled DNAfs (D) and Cfs (E) with chromosomes of treated cells. NIH3T3 cells were treated with labelled DNAfs and Cfs and metaphase spreads were prepared 6 h after treatment and analysed by fluorescence microscopy. Note that the labelled DNA particles are considerably smaller in size than Cfs particles.

Article Snippet: RNA was quantified using Nanodrop 1000 spectrophotometer (Thermo Scientific, USA) and labelled using ULS™ microRNA Labeling Kit (With Cy3) (Kreatech Diagnostics, The Netherlands.

Techniques: Fluorescence, Microscopy